Expression Levels of PPARγ and CYP-19 in Polycystic Ovarian Syndrome Primary Granulosa Cells: Influence of ω-3 Fatty Acid
نویسندگان
چکیده مقاله:
Background The omega-3 fatty acid (ω-3 fatty acid) such as eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement in the treatment of polycystic ovarian syndrome (PCOS). The present study was designed to investigate the effect of EPA on the expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and cytochrome P450 aromatase (encoded by the CYP-19) in primary cultured granulosa cells (GC) from patients undergoing in vitro fertilization (IVF), and also to compare these effects with those in GC of PCOS patients. MaterialsAndMethods In this experimental study, human GC were isolated, primary cultured in vitro, exposed to a range of concentrations of the EPA and investigated with respect to gene expression levels of PPARγ and CYP-19 using real time-polymerase chain reaction (PCR). The participants (n=30) were the patients admitted to the IVF Center in February-March 2013 at Alzahra Hospital, Tabriz, Iran, who were divided into two groups as PCOS (n=15) and non-PCOS (n=15) women (controls). Results All doses of the EPA significantly induced PPARγ mRNA gene expression level as compared to the control recombinant follicle stimulating hormone (rFSH) alone condition. High doses of EPA in the presence of rFSH produced a stimulatory effect on expression level of PPARγ (2.15-fold, P=0.001) and a suppressive effect (0.56-fold, P=0.01) on the expression level of CYP-19, only in the PCOS GC. Conclusion EPA and FSH signaling pathway affect differentially on the gene expression levels of PPARγ and CYP-19 in PCOS GC. Altered FSH-induced PPARγ activity in PCOS GC may modulate the CYP-19 gene expression in response to EPA, and possibly modulates the subsequent steroidogenesis of these cells.
منابع مشابه
expression levels of pparγ and cyp-19 in polycystic ovarian syndrome primary granulosa cells: influence of ω-3 fatty acid
background: the omega-3 fatty acid (ω-3 fatty acid) such as eicosapentaenoic acid (epa) is currently used in the clinic as a nutritional supplement in the treatment of polycystic ovarian syndrome (pcos). the present study was designed to investigate the effect of epa on the expression levels of peroxisome proliferator-activated receptor gamma (pparγ) and cytochrome p450 aromatase (encoded by th...
متن کاملExpression Levels of PPARγ and CYP-19 in Polycystic Ovarian Syndrome Primary Granulosa Cells: Influence of ω-3 Fatty Acid
BACKGROUND The omega-3 fatty acid (ω-3 fatty acid) such as eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement in the treatment of poly- cystic ovarian syndrome (PCOS). The present study was designed to investigate the ef- fect of EPA on the expression levels of peroxisome proliferator-activated receptor gamma (PPARγ) and cytochrome P450 aromatase (encoded by...
متن کاملinfluence of ω-3 fatty acid eicosapentaenoic acid on igf-1 and cox-2 gene expression in granulosa cells of pcos women
background: the omega-3 (ω-3) fatty acid eicosapentaenoic acid (epa) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (pcos).objective: the present study was designed to investigate the effect of epa on insulin-like growth factor 1 (igf-1) and cyclooxygenase 2 (cox-2) gene expression in primary cultured gr...
متن کاملP-200: Effects of The ?3 Fatty Acid Eicosapentaenoic Acid on PPARa and Aromatase in Human Granulosa Cells
متن کامل
Influence of ω-3 fatty acid eicosapentaenoic acid on IGF-1 and COX-2 gene expression in granulosa cells of PCOS women
BACKGROUND The omega-3 (ω-3) fatty acid eicosapentaenoic acid (EPA) is currently used in the clinic as a nutritional supplement to improve infertility, particularly in women with polycystic ovarian syndrome (PCOS). OBJECTIVE The present study was designed to investigate the effect of EPA on insulin-like growth factor 1 (IGF-1) and cyclooxygenase 2 (COX-2) gene expression in primary cultured g...
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عنوان ژورنال
دوره 9 شماره 2
صفحات 197- 204
تاریخ انتشار 2015-07-01
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